colorimetric kit  (Elabscience Biotechnology)

Journal: Pharmaceuticals

Article Title: Scutellarein from Erigeron breviscapus Inhibits Apoptosis-Mediated Epithelial Barrier Disruption and Alleviates Cigarette Smoke-Induced Lung Injury

doi: 10.3390/ph19010113

Figure Lengend Snippet: Scu reduces CS-induced inflammatory responses in BALF and lung tissue. Quantification of leukocytes ( A ), neutrophils ( B ), lymphocytes ( C ), and monocytes ( D ) in BALF from FA or CS-exposed mice receiving Veh (0.3% ( w / v ) CMC-Na solution containing 0.2% ( v / v ) Tween-80 and 0.2% ( v / v ) DMSO), 5, 10, 15 mg/kg Scu or 5 mg/kg Dex. Quantification of the contents of IL-1β ( E ), CXCL1 ( F ), TNF-α ( G ), and MCP-1 ( H ) in BALF from mice treated as in ( A – D ). Relative mRNA expression levels of Il1b ( I ), Cxcl1 ( J ), Tnf ( K ), Mcp-1 ( L ), and Mmp-9 ( M ) in lung tissue samples collected from differently treated mice. ( N ) Quantification of myeloperoxidase (MPO) in lung tissue samples collected from differently treated mice. Data represent mean ± SEM. N = 6; *, p < 0.05, **, p < 0.01, vs. FA-Veh; #, p < 0.05, ##, p < 0.01, vs. CS-Veh, by one-way ANOVA with Bonferroni’s multiple comparisons tests.

Article Snippet: Myeloperoxidase (MPO) activity detection kit (Cat# A114-1-1) was sourced from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Techniques: Expressing